Rabbit IFN-gamma Recombinant Monoclonal Antibody

SKU: orb1806421

Description

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IHC, IP, WB
Dilution Range	Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR300L
Immunogen	Residues 23-166
Target	IFN-gamma
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFG;IFI;IFN-gamma;immune interferon;interferon gamma

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Rabbit IFN-gamma Recombinant Monoclonal Antibody

Detection of human IFN-gamma by western blot. Samples: Whole cell lysate (50 µg) from NK-92 cells untreated (-) and treated (+) with 80nM TPA, 3µM Ionomycin, 300ng/mL Brefeldin A. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human IFN-gamma by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human IFN-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells treated with 80nM TPA, 3µM Ionomycin, and 300ng/mL Brefeldin A. Antibodies: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421) used for IP at 6 µl/mg lysate.

Detection of human IFN-gamma by immunocytochemistry. Sample: FFPE section of NK-92 cells treated with TPA, Ionomycin, Brefeldin A (left) and mock treated NK-92 cells (right). Antibody: Rabbit anti-IFN-gamma recombinant monoclonal antibody (orb1806421). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

IFN-gamma

UniProt

P01579

RefSeq (Protein):NP_000610.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000610.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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