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Rabbit IL-4 Recombinant Monoclonal Antibody
SKU: orb1806424
Description
Research Area
Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | Application : IP , ApplicationDilutionRange : 20 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR298L |
| Immunogen | Residues 25-153 |
| Target | IL-4 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B cell growth factor 1;B_cell stimulatory factor 1;B-cell stimulatory factor 1;BCGF1;BCGF-1;binetrakin;BSF1;BSF-1;IL-4;interleukin 4 variant 2;interleukin-4;lymphocyte stimulatory factor 1;pitrakinra
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Detection of human IL-4 by western blot. Samples: Human recombinant IL-4 (0.1 µg) protein. Antibody: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806424) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human IL-4 by western blot. Samples: Whole cell lysate (10 µg) from 293-F untransfected and IL-4 overexpressing (hOE) cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806424) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human IL-4 by western blot of immunoprecipitates. Samples: Whole cell lysate (500 µg per IP; 5% of IP loaded) from 293F cells overexpressing human IL-4. Antibodies: Rabbit anti-IL-4 recombinant monoclonal antibody (orb1806424) used for IP at 20 µl/mg lysate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000580.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit IL-4 Recombinant Monoclonal Antibody (orb1806424)
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