Rabbit INSM1 Recombinant Monoclonal Antibody

SKU: orb1733233

Description

INSM1 Antibody

Research Area

Neuroscience

Images & Validation

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Item 1 of 6

Tested Applications	ICC, IHC, WB
Dilution Range	Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR272L
Immunogen	Between 75 and 125
Target	INSM1
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IA1; IA-1; insulinoma-associated 1; insulinoma-associated protein 1; zinc finger protein IA-1

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Rabbit INSM1 Recombinant Monoclonal Antibody

Detection of human INSM1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, NCI-H1155, HeLa, SHP-77, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse INSM1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, Beta-TC-6, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of neuroendocrine tumor. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of human pancreas. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse INSM1 by immunocytochemistry. Sample: FFPE section of Beta-TC-6 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

INSM1

UniProt

Q01101

RefSeq (Protein):NP_002187.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002187.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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