Rabbit JAM-A Antibody

SKU: orb1525815

Description

JAM-A Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 249 and C-terminus
Target	JAM-A
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD antigen CD321; CD321; JAM; JAM1; JAM-1; JAMA; JAM-A; JCAM; junctional adhesion molecule 1; junctional adhesion molecule A; KAT; PAM-1; platelet adhesion molecule 1; platelet F11 receptor

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Detection of human JAM-A by western blot. Samples: Whole cell lysate from HEK293T (5, 15, and 50 µg) and HeLa (H; 50 µg) cells. Antibody: Affinity purified rabbit anti-JAM-A antibody orb1525815 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human JAM-A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JAM-A antibody orb1525815 used for IP at 3 µg/mg lysate.

Detection of human JAM-A by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAM-A (Cat. No. orb1525815) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

JAM-A

UniProt

Q9Y624

RefSeq (Protein):NP_058642.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_058642.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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