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Rabbit KLF5 Recombinant Monoclonal Antibody
SKU: orb1733253
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 9
| Tested Applications | FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.1 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR243L |
| Immunogen | Between 325 and 375 |
| Target | KLF5 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTEB2; BTE-binding protein 2; CKLF; colon krueppel-like factor; colon kruppel-like factor; epididymis secretory sperm binding protein; GC box binding protein 2; GC-box-binding protein 2; IKLF; intestinal Kruppel-like factor; intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; Klf5C isoform; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); transcription factor BTEB2
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Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used for IP at 6 µl/mg lysate.
Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001721.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit KLF5 Recombinant Monoclonal Antibody (orb1733253)
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