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Rabbit LAG3 Recombinant Monoclonal Antibody
SKU: orb1806464
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR261L |
| Immunogen | Between 250 and 300 |
| Target | LAG3 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD antigen CD223;CD223;LAG-3;Ly66;lymphocyte activation gene 3 protein
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Detection of mouse LAG3 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, CTLL-2, NIH 3T3, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse LAG3 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of CTLL-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse LAG3 by immunocytochemistry. Sample: FFPE section of J774A.1 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1806464). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_032505.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Rabbit LAG3 Recombinant Monoclonal Antibody (orb1806464)
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