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Rabbit MEPCE Antibody
SKU: orb1523470
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 200 and 250 |
| Target | MEPCE |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−7SK snRNA methylphosphate capping enzyme; BCDIN3; bicoid-interacting protein 3 homolog; bin3 homolog; bin3, bicoid-interacting 3, homolog; MePCE
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Detection of human and mouse MEPCE by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody orb1523470 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MEPCE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody orb1523470 used for IP at 6 µg/mg lysate.
Detection of human MEPCE by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEPCE (Cat. No. orb1523470) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_062552.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit MEPCE Antibody (orb1523470)
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