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Rabbit anti-Myc Tag Recombinant Monoclonal Antibody
SKU: orb1530834
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, ICC, IP, WB |
|---|---|
| Dilution range | WB - 1:1000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:50,000; for coating plates 1:100 - 1:500 |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLRE01G |
| Immunogen | Residues 410-419 (EQKLISEEDL) of human myc |
| Target | Myc Tag |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Disclaimer | For research use only |
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Detection of Myc-tagged protein by WB from non-transfected human HEK293 cells, HEK293 transfected with Met-Myc-IDO1, HEK293 with IDO1-Myc (C-terminal Tag), and HEK293 with IDO1-Myc-HIS (Internal Tag), using antibody at 1:1000.
Detection of Myc Tag by western blot of immunoprecipitates from HEK293 transfected with Met-Myc-IDO1 lysate.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.
Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.
Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged nuclear protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.
Quick Database Links
Gene Symbol
Myc Tag
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Rabbit anti-Myc Tag Recombinant Monoclonal Antibody (orb1530834)
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