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Rabbit Myc Tag Recombinant Monoclonal Antibody

SKU: orb1530834

Description

Myc Tag Antibody

Images & Validation

Tested ApplicationsELISA, ICC, IP, WB
Dilution RangeWB - 1:1000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:50,000; for coating plates 1:100 - 1:500
Application Notes
Format: Whole IgG

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.BLRE01G
ImmunogenResidues 410-419 (EQKLISEEDL) of human myc
TargetMyc Tag
PurificationPurified
ConjugationUnconjugated

Storage & Handling

Storage2 - 8°C
Form/AppearanceLiquid
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration250 µg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Rabbit Myc Tag Recombinant Monoclonal Antibody

Detection of Myc-tagged protein by WB from non-transfected human HEK293 cells, HEK293 transfected with Met-Myc-IDO1, HEK293 with IDO1-Myc (C-terminal Tag), and HEK293 with IDO1-Myc-HIS (Internal Tag), using antibody at 1:1000.

Rabbit Myc Tag Recombinant Monoclonal Antibody

Detection of Myc Tag by western blot of immunoprecipitates from HEK293 transfected with Met-Myc-IDO1 lysate.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.

Rabbit Myc Tag Recombinant Monoclonal Antibody

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.

Rabbit Myc Tag Recombinant Monoclonal Antibody

Detection of Myc Tag in FFPE HEK293 cells expressing Myc-tagged nuclear protein by ICC.Antibody: Rabbit anti-Myc Tag recombinant monoclonal antibody.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Rabbit Myc Tag Recombinant Monoclonal Antibody (orb1530834)

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100 μl
$ 470.00
DispatchUsually dispatched within 5-10 working days
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