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Rabbit NCBP2 Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 106 and C-terminus |
| Target | NCBP2 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CBP20 rabbit pAb Antibody [orb764757]
ELISA, IF, IHC, WB
Human, Mouse
Polyclonal
Unconjugated
100 μl, 50 μlRabbit NCBP2 Antibody [orb1526424]
IHC, IP
Bovine, Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 10 μl

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Detection of human and mouse NCBP2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NCBP2 antibody orb1526422 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human and mouse NCBP2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-NCBP2 (Cat. No. orb1526422) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1 µg/ml). Detection: DAB
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_031388.2 |
|---|
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Protocol Information
Rabbit NCBP2 Antibody (orb1526422)
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