Rabbit NF110 Antibody

SKU: orb1525422

This product can be custom made

Description

NF110 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 844 and C-terminus
Target	NF110
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer-binding factor 3; MMP4; M-phase phosphoprotein 4; MPHOSPH4; MPP4; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1; NFAR2; NF-AT-90; nuclear factor associated with dsRNA; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; translational control protein 80

Quality Guarantee

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Detection of human NF110 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody orb1525422 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of mouse NF110 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NF110 antibody orb1525422 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NF110 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF110 antibody orb1525422 used for IP at 6 µg per reaction.

Detection of human NF110 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NF110 (Cat. No. orb1525422) used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NF110

UniProt

Q12906

RefSeq (Protein):NP_703194.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_703194.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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