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Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | NF90/NF110 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer-binding factor 3; MMP4; M-phase phosphoprotein 4; MPHOSPH4; MPP4; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1; NFAR2; NF-AT-90; nuclear factor associated with dsRNA; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; translational control protein 80
Similar Products
−- Item 1 of 2
Rabbit NF90/NF110 Antibody [orb1525424]
IHC, WB
Rat
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl, 10 μl - Item 1 of 1
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Detection of human and mouse NF90 and NF110 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF90/NF110 antibody orb1525428 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies as well as anti-NF110 antibody, each of which recognizes a different downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Detection of mouse NF90 and NF110 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-NF90/NF110 (Cat. No. orb1525428) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_703194.1 |
|---|
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Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Rabbit NF90/NF110 Antibody (orb1525428)
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