Rabbit NIR Antibody

SKU: orb1522833

Description

NIR Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 699 and C-terminus
Target	NIR
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NET15; NET7; NIR; NOC2L nucleolar associated transcriptional repressor; NOC2-like protein; novel INHAT (inhibitor of histone acetyltransferase) repressor; novel INHAT repressor; nucleolar complex associated 2 homolog; nucleolar complex protein 2 homolog; PPP1R112; protein NOC2 homolog; protein phosphatase 1, regulatory subunit 12

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Detection of human NIR by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIR antibody orb1522833 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NIR by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NIR (Cat. No. orb1522833) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Quick Database Links

Gene Symbol

NIR

UniProt

Q9Y3T9

RefSeq (Protein):NP_056473.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_056473.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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