Rabbit NOB1 Antibody

SKU: orb1522535

Description

NOB1 Antibody

Images & Validation

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Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 362 and C-terminus
Target	NOB1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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adenocarcinoma antigen recognized by T lymphocytes 4; ART-4; MST158; MSTP158; nin one binding protein; NIN1/PSMD8 binding protein 1 homolog; NOB1P; phosphorylation regulatory protein HP-10; protein ART-4; PSMD8 binding protein 1; PSMD8BP1; RNA-binding protein NOB1

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Detection of human and mouse NOB1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOB1 antibody orb1522535 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human NOB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOB1 antibody orb1522535 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

NOB1

UniProt

Q9ULX3

RefSeq (Protein):NP_054781.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_054781.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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