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Rabbit OTULIN Antibody
SKU: orb1520375
Description
Research Area
Cancer Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 75 and 125 |
| Target | OTULIN |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−AIPDS; deubiquitinating enzyme otulin; FAM105B; family with sequence similarity 105, member B; GUM; OTU domain-containing deubiquitinase with linear linkage specificity; ubiquitin thioesterase Gumby; ubiquitin thioesterase otulin
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Detection of human and mouse OTULIN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, U2OS, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-OTULIN antibody (orb1520375) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human OTULIN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTULIN antibody (orb1520375) used for IP at 2 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_612357.4 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit OTULIN Antibody (orb1520375)
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