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Rabbit PAF Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP |
|---|---|
| Dilution range | WB - Not recommended. Use rabbit anti-PAF antibody A304-374A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | PAF |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
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Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody orb1523114 used for IP at 6 µg per reaction.

Detection of human PAF by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. orb1523114) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_055551.1 |
|---|
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Protocol Information
Rabbit PAF Antibody (orb1523114)
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