Rabbit PBRM1 Recombinant Monoclonal Antibody

SKU: orb1519955

Description

PBRM1 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC, IHC, IP, WB
Dilution Range	WB - 1:1000; IP - 5 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BL-39-2C3
Immunogen	Residues 1-293
Target	PBRM1
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	250 µg/ml
Disclaimer	For research use only

Alternative Names

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BAF180; BRG1-associated factor 180; hPB1; PB1; polybromo-1D; protein polybromo-1

Quality Guarantee

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Rabbit PBRM1 Recombinant Monoclonal Antibody

Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (orb1519955). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.

Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (orb1519955) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Quick Database Links

Gene Symbol

PBRM1

UniProt

Q86U86

RefSeq (Protein):NP_060783.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_060783.3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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