Rabbit PCNA Antibody

SKU: orb1530430

Description

PCNA Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:5,000 - 1:20,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:1,000 - 1:5,000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 211 and C-terminus
Target	PCNA
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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ATLD2; cyclin; DNA polymerase delta auxiliary protein; PCNA; proliferating cell nuclear antigen

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Detection of mouse PCNA by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.

Detection of human PCNA by western blot.Samples: Whole cell lysate from A-549, Jurkat, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.

Detection of mouse PCNA by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).

Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).

Detection of human PCNA by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-PCNA antibody.

Detection of human PCNA by IHC. Sample: human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA at 1:4, 000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_002583.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002583.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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