Rabbit PCNA Antibody

SKU: orb1530432

Description

PCNA Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC, IP, WB
Dilution Range	WB - 1:1,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Gallus, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 75 and 125
Target	PCNA
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATLD2; cyclin; DNA polymerase delta auxiliary protein; PCNA; proliferating cell nuclear antigen

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Rabbit PCNA Antibody [orb1530430]
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IHC, IP, WB
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Bovine, Rat
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Human, Mouse
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Rabbit
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Polyclonal
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Unconjugated
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Detection of human and mouse PCNA by western blot.Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody orb1530432 at 0.1 µg/ml.

Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:10, 000 (0.1µg/ml).

Detection of human PCNA by WB and IP.Samples: A. A-431 cells. B. MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody used at the indicated concentrations for WB.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_002583.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002583.1

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

Flow Cytometry

Immunoprecipitation