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Rabbit Phospho 53BP1 (S25) Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 1 - 4 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding serine 25 |
| Target | 53BP1 Phospho (S25) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of Phospho 53BP1 (S25) by western blot.Samples: HEK293T cells that were mock treated or treated with NCS. Antibodies: Affinity purified rabbit anti-Phospho 53BP1 antibody orb1529849 at 0.04 µg/ml (A) and 0.1 µg/ml (B) for WB.

Detection of human and mouse Phospho 53BP1 (S25) by immunohistochemistry. Sample: FFPE sections of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Phospho 53bp1 (S25) used at a dilution of 1:1, 000.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005648.1 |
|---|
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Protocol Information
Rabbit Phospho 53BP1 (S25) Antibody (orb1529849)
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