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Rabbit Phospho KAP-1 (S824) Antibody
SKU: orb1523532
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Surrounding serine 824 |
| Target | KAP-1 Phospho (S824) |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−AA408787; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB-A-interacting protein; KRIP-1; MommeD9; RING-type E3 ubiquitin transferase TIF1-beta; Tif1b; Tif1beta; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; tripartite motif protein 28; tripartite motif-containing protein 28
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Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 µg) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody orb1523532 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti- KAP-1 antibody used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_035718.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit Phospho KAP-1 (S824) Antibody (orb1523532)
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