Rabbit Phospho KAP-1 (S824) Antibody

SKU: orb1529675

Description

KAP-1, Phospho (S824) Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	ICC, IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:200 - 1:1,000; ICC-IF - 1:200 - 1:1,000
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Surrounding serine 824
Target	KAP-1 Phospho (S824)
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB Kruppel-associated box domain-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1B; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; Tripartite motif-containing protein 28

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Detection of human phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of mouse phospho KAP-1 (S824) by western blot.Samples: Whole cell lysate from NIH 3T3 cells treated with 100 µM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody at 0.04 µg/ml.

Detection of human Phospho KAP-1 (S824) by IHC.Samples: FFPE serial sections of prostate carcinoma with MOCK CIP treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at a dilution of 1:200.

Detection of human phospho KAP-1 (S824) by western blot of immunoprecipitates.Samples: HEK293T cells. Antibodies: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody and rabbit anti-KAP-1 recombinant monoclonal antibody.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells with MOCK CIP treatment (left) and with CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) at 1:200.

Detection of human Phospho KAP-1 (S824) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200. Detection: Red fluorescent anti-rabbit IgG-DyLight® 594 conjugated.

Quick Database Links

Gene Symbol

KAP-1 Phospho (S824)

UniProt

Q13263

RefSeq (Protein):NP_005753.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005753.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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