Rabbit Pol Lambda Antibody

SKU: orb1528045

Description

Pol Lambda Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 175 and 225
Target	Pol Lambda
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BETAN; DNA polymerase beta-2; DNA polymerase beta-N; DNA polymerase kappa; DNA polymerase lambda; Pol beta2; Pol Lambda; POLKAPPA; polymerase (DNA directed), lambda; polymerase (DNA) lambda

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Detection of human Pol Lambda by western blot. Samples: Whole cell lysate (50 µg) from U2OS, GaMG, HeLa, HEK293T, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pol Lambda antibody (orb1528045) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody (orb1528045) used for IP at 6 µg per reaction. Pol Lambda was also immunoprecipitated by a previous lot of this antibody (orb1528045) and a second antibody against a different epitope of Pol Lambda (orb1528043).

Quick Database Links

Gene Symbol

Pol Lambda

UniProt

Q9UGP5

RefSeq (Protein):NP_037406.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_037406.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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