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Rabbit PolD1 Antibody
SKU: orb1523767
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 850 and 900 |
| Target | PolD1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDC2; CDC2 homolog; CRCS10; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; MDPL; POLD; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit
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Detection of human and mouse PolD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PolD1 antibody orb1523767 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PolD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolD1 antibody orb1523767 used for IP at 6 µg/mg lysate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002682.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit PolD1 Antibody (orb1523767)
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