Rabbit PSMD1 Antibody

SKU: orb1524063

Description

PSMD1 Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1 and 50
Target	PSMD1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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26S proteasome non-ATPase regulatory subunit 1; 26S proteasome regulatory subunit RPN2; 26S proteasome regulatory subunit S1; 26S proteasome subunit p112; P112; proteasome (prosome, macropain) 26S subunit, non-ATPase, 1; Rpn2; S1

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Detection of human PSMD1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (H; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD1 antibody orb1524063 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PSMD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMD1 antibody orb1524063 used for IP at 6 µg/mg lysate.

Detection of human PSMD1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PSMD1 (Cat. No. orb1524063) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PSMD1

UniProt

Q99460

RefSeq (Protein):NP_002798.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002798.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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