Rabbit anti-PU.1 Recombinant Monoclonal Antibody

SKU: orb1519544

Description

PU.1 Antibody

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB - 1:1000; IP - 12 µl/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR178J
Immunogen	Between 1 and 50
Target	PU.1
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	500 µg/ml
Disclaimer	For research use only

Alternative Names

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SFPI1; transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; 31 kDa transforming protein; SPI-1; PU.1; OF; hematopoietic transcription factor PU.1; 31 kDa-transforming protein; SPI-A

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Detection of human PU.1 (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (orb1519544) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

PU.1

UniProt

P17947

RefSeq (Protein):NP_003111.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003111.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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