Rabbit PVRL1/NECTIN1 Recombinant Monoclonal Antibody

SKU: orb1784567

Description

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	FC, IP, WB
Dilution Range	Application : Flow-Cyt , ApplicationDilutionRange : Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR274L
Immunogen	Residues 31-331 (ECD)
Target	PVRL1/NECTIN1
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD antigen CD111;CD111;CLPED1;ectodermal dysplasia 4 (Margarita Island type);ED4;herpes simplex virus type 1 sensitivity;herpes virus entry mediator C;Herpesvirus entry mediator C;herpesvirus Ig-like receptor;HIgR;HV1S;HVEC;nectin 1;Nectin cell adhesion molecule 1;nectin-1;OFC7;poliovirus receptor-like 1;poliovirus receptor-related 1 (herpesvirus entry mediator C);poliovirus receptor-related protein 1;PRR;PRR1;PVRL1;PVRR;PVRR1;SK-12

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Rabbit PVRL1/NECTIN1 Recombinant Monoclonal Antibody

Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.

Quick Database Links

Gene Symbol

PVRL1/NECTIN1

UniProt

Q15223

RefSeq (Protein):NP_002846.3

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002846.3

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation