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Rabbit Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human serum Antibody

SKU: orb700247

Description

Rabbit polyclonal antibody against Rabbit anti-Rat IgG (H&L) conjugated to HRP

Images & Validation

Dilution RangeThis conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
ReactivityRat
Application Notes
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins

Key Properties

HostRabbit
ClonalityPolyclonal
ImmunogenPurified rat IgG (H&L)
PurificationImmunogen affinity purified rabbit IgG.
ConjugationHRP

Storage & Handling

StorageStore lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/AppearanceLyophilized
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Rabbit Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human serum Antibody (orb700247)

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