Rabbit SOX10 Recombinant Monoclonal Antibody

SKU: orb1519802

Featured

Description

SOX10 Antibody

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

−

Item 1 of 4

Tested Applications	ICC, IF, IHC, IP, WB
Dilution Range	(ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
Reactivity	Human, Mouse
Application Notes	All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR080G
Immunogen	Between 416 and C-terminus
Target	SOX10
Purity	Recombinant antibody was purified from cell culture supernatant
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	50 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

DOM; dominant megacolon, mouse, human homolog of; PCWH; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS2E; WS4; WS4C

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rabbit SOX10 Recombinant Monoclonal Antibody

Detection of human SOX10 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (orb1519802). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, Malme-3M, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH

Detection of human SOX10 in FFPE melanoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human SOX10 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Quick Database Links

Gene Symbol

SOX10

UniProt

P56693

RefSeq (Protein):NP_008872.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_008872.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

ICC

Immunocytochemistry

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols