Rabbit TAF1 Antibody

SKU: orb1524711

Description

TAF1 Antibody

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1800 and 1850
Target	TAF1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BA2R; CCG1; CCGS; cell cycle gene 1 protein; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; DYT3; DYT3/TAF1; KAT4; MRXS33; NSCL2; N-TAF1; OF; P250; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; TAF2A; TAFII250; TAFII-250; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; Transcription initiation factor TFIID 250 kDa subunit; transcription initiation factor TFIID subunit 1; XDP

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Detection of human TAF1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody orb1524711 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody orb1524711 used for IP at 6 µg per reaction.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of lung carcinoma.Antibody: Rabbit anti-TAF1 antibody (orb1524711) used at 1: 5000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of bladder carcinoma. Antibody: Rabbit anti-TAF1 antibody (orb1524711) used at 1: 1000 (1 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Quick Database Links

Gene Symbol

TAF1

UniProt

P21675

RefSeq (Protein):NP_620278.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_620278.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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