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Rabbit Troponin I Recombinant Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR280L |
| Immunogen | Between 1 and 50 |
| Target | Troponin I |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rabbit Troponin I Recombinant Monoclonal Antibody [orb1806456]
IHC, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μg (BSA-free)TNNI2 Rabbit Monoclonal Antibody [orb2988794]
WB
Human, Mouse, Rat
Rabbit
Monoclonal
Unconjugated
200 μl, 100 μl, 50 μl, 30 μl

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Detection of mouse Troponin I by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, heart, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.

Detection of human Troponin I by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, heart, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH antibody.

Detection of human Troponin I by immunohistochemistry. Sample: FFPE section of human cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse Troponin I by immunohistochemistry. Sample: FFPE section of mouse cardiac muscle. Antibody: Rabbit anti-Troponin I recombinant monoclonal antibody (orb1806457). Secondary: HRP-conjugated goat anti-rabbit IgG.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000354.4 |
|---|
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Protocol Information
Rabbit Troponin I Recombinant Monoclonal Antibody (orb1806457)
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