Rabbit V5 Tag Antibody (HRP)

SKU: orb1530858

Description

V5 Tag Antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, ICC, WB
Dilution Range	WB - 1:1,000 - 1:30,000; IHC - Not applicable; ICC - 1:200 - 1:500; ELISA - 1:10,000 - 1:100,000
Application Notes	Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target	V5 Tag
Purification	Antigen Affinity Purified
Conjugation	HRP

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Phosphate Buffered Saline (PBS) containing 0.2% rAlbumin and 0.05% Pro-Clean 400
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Detection of V5-tagged protein by western blot.Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-V5 antibody orb1530858 at 0.2 µg/ml (1:5, 000).

Quick Database Links

Gene Symbol

V5 Tag

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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