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Rabbit V5 Tag Recombinant Monoclonal Antibody
SKU: orb1530832
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ICC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLRE03H |
| Immunogen | V5 Epitope tag (GKPIPNPLLGLDST) |
| Target | V5 Tag |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 250 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
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Detection of V5-tagged protein by WB from HEK293 transfected with Met-V5-IDO1, HEK293 with IDO1-V5-HA (Internal Tag), and HEK293 with IDO1-HA-V5 (C-terminal Tag) cells, using Rabbit anti-V5 Tag recombinant monoclonal antibody.
Detection of V5 Tag by western blot of immunoprecipitates from HEK293 transfected with IDO1-V5.Antibodies: Antibodies: Rabbit anti-V5 recombinant monoclonal antibody.
Detection of V5 tag in FFPE HEK293 cells expressing V5 tagged nuclear protein by ICC.Antibody: Rabbit anti-V5 tag recombinant monoclonal antibody.
Detection of V5 tag in FFPE HEK293 cells expressing V5 tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-V5 tag recombinant monoclonal antibody.
Quick Database Links
Gene Symbol
V5 Tag
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit V5 Tag Recombinant Monoclonal Antibody (orb1530832)
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