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Rabbit VE-Cadherin Recombinant Monoclonal Antibody
SKU: orb1566962
Description
Research Area
Cardiovascular Research, Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1,000; IP - 12 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BLR239K |
| Immunogen | Residues 25-595 (ECD) |
| Target | VE-Cadherin |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 500 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−7B4; 7B4/cadherin-5; AA408225; cadherin-5; CD antigen CD144; Cd144; vascular endothelial cadherin; Vec; VEcad; VE-Cad; VE-cadherin; VECD
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Detection of mouse VE-Cadherin by western blot. Samples: Whole cell lysate (2 µg) from NIH 3T3, CT26, bEnd.3, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody (orb1566962) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_033998.2 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Rabbit VE-Cadherin Recombinant Monoclonal Antibody (orb1566962)
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