Rabbit IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb347690

Description

Rabbit IgG F(c) antibody (Alkaline Phosphatase)

Images & Validation

−

Item 1 of 2

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Rabbit
Application Notes	Anti-Rabbit IgG F(c) alkaline phosphatase antibody has been tested by ELISA and dot blot and is assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product.

Key Properties

−

Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Rabbit IgG F(c) fragment
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab)or Human Serum Proteins.
Conjugation	AP

Storage & Handling

−

Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.05% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

Goat anti-Rabbit IgG F(c) Antibody alkaline phosphatase Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody alkaline phosphatase Conjugation, Goat anti-Rabbit IgG F(c) alk phos Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rabbit IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Dot Blot of Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT.

Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n orb347661). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n orb347661) 1:2000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

DOT

Dot Blot

View Protocol

Find More Protocols