Rabbit IgG Fc Antibody Rhodamine Conjugated

(a) Channels coating: remnant fluorescence due to nonspecific binding of anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] at a concentration of 100 µg/ml in uncoated and coated microchannels with silane-PEG. (b) Fluorescence obtained from the complete immunoassay at a flow rate of 5 µl/h and at a anti-biotin rabbit IgG (p/n orb750609) [AbI] concentration of 50 pg/ml is compared to the fluorescence obtained from the immunoassay performed without applying the magnetic field (column 1), without adding anti-biotin rabbit IgG (p/n orb750609) [AbI] (column 2), without adding), anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] (column 3), and, finally, the efficacy of the microparticles coating was tested by performing the immunoassay without anti-biotin rabbit IgG (p/n orb750609) [AbI] and with noncoated microparticles (column 5). The level of fluorescence of the two first columns in b results from nonspecific interactions of anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] with the microparticles. Error bars are standard deviation.