Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347768

Description

RABBIT IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Rabbit
Application Notes	Anti-RABBIT IgG Alkaline Phosphatase Conjugated Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG
Immunogen	Rabbit IgG whole molecule
Purity	Anti-RABBIT IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Donkey anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation

Quality Guarantee

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Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n orb347767). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n orb347767) 1:5000 for 60 min at RT. Secondary antibody: None. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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