Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated

SKU: orb347673

Description

RABBIT IgG (H&L) antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:5,000 - 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:10,000 - 1:20,000
Reactivity	Rabbit
Application Notes	Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. 0.384 moles Peroxidase (HRP) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Rabbit IgG whole molecule
Purity	RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Conjugation	HRP

Storage & Handling

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Storage	Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	2 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat anti-Rabbit IgG Secondary Antibody Peroxidase Conjugated, Goat anti-Rabbit IgG Secondary Antibody HRP Conjugated, GAR-HRP, Anti-rabbit secondary antibody, anti-rabbit HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-rabbit horseradish peroxidase conjugated secondary antibody

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Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated

ELISA results of Goat Anti-Rabbit IgG Antibody Peroxidase Conjugated tested against purified Rabbit IgG protein. Each well was coated in duplicate with 10 µg of Rabbit IgG (p/n orb2652747). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:98500. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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