Rabbit Isotype Control Polyclonal Antibody

SKU: orb248101

Description

Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.

Images & Validation

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Tested Applications	FACS, IF, IHC
Dilution range	FACS: assay dependent,IHC: assay dependent,IF: assay dependent
Application Notes	Generally to be used at the same concentration as the sample being controlled for.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This is purified naive rabbit sera and as such there was no immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1X PBS, pH 7.4
Disclaimer	For research use only

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Rabbit Isotype Control Polyclonal Antibody

FACS testing of Rabbit IgG isotype control antibody on human samples. Black = cells alone, gray = cells + secondary, orange = cells + isotype control antibody + secondary.

FACS testing of Rabbit IgG isotype control antibody on mouse samples. Black = cells alone, gray = cells + secondary, orange = cells + isotype control antibody + secondary

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Protocol Information

IHC

Immunohistochemistry

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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