Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

SKU: orb780018

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A(CDKN1A).

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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WAF1 ELISA Kit, p21 ELISA Kit, CIP1 ELISA Kit, p21WAF1 ELISA Kit, CAP20 ELISA Kit, CDKN1 ELISA Kit, MDA-6 ELISA Kit, SDI1 ELISA Kit, p21CIP1 ELISA Kit, CDK-Interacting Protein 1 ELISA Kit, Melanoma differentiation-associated protein 6 ELISA Kit

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Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Rat (1)

Reham L Younis 1, Rehab M El-Gohary 2, Asmaa A Ghalwash 2, Islam Ibrahim Hegab 1 3, Maram M Ghabrial 4, Azza M Aboshanady 4, Raghad A Mostafa 5, Alaa H Abd El-Azeem 6, Eman E Farghal 5, Asmaa A E Belal 7, Haidy Khattab Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection Neurochem Res, (2024)

PubMed: 38987448

Applications

ELISA

Reactivity

Rat