Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

SKU: orb778848

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 2(ERK2).

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ERK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ERK2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.061 ng/mL
Concentration	10 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

MAPK1 ELISA Kit, P38 ELISA Kit, P40 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, P42-MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, P41 ELISA Kit, P41mapk ELISA Kit, P41-mapk ELISA Kit, Mitogen-Activated Protein Kinase 1 ELISA Kit, Mitogen-activated protein kinase 2 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

Quick Database Links

UniProt

P63086

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols