Rat Follicle Stimulating Hormone (FSH) ELISA Kit

SKU: orb775180

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Follicle Stimulating Hormone(FSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Follicle Stimulating Hormone(FSH).

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Tested Applications

ELISA

Application Notes

standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FSH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	0.91 ng/mL
Concentration	100 mIU/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Follicle Stimulating Hormone ELISA Kit

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Rat Follicle Stimulating Hormone (FSH) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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