Rat Gonadotropin Releasing Hormone (GnRH) ELISA Kit

SKU: orb779304

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gonadotropin Releasing Hormone(GnRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gonadotropin Releasing Hormone(GnRH).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GnRH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	15.63-1000 pg/mL
Sensitivity	5.13 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GNRH1 ELISA Kit, GRH ELISA Kit, LNRH ELISA Kit, LHRH ELISA Kit, LH-RH I ELISA Kit, Luliberin ELISA Kit, Luteinizing-Hormone Releasing Hormone ELISA Kit, Progonadoliberin-1 ELISA Kit, Gonadoliberin I ELISA Kit, Gonadorelin ELISA Kit, GnRH-associated peptide 1 ELISA Kit

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Rat Gonadotropin Releasing Hormone (GnRH) ELISA Kit

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UniProt

P07490

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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