Rat IgG F(ab')2 Antibody

SKU: orb346339

Description

Research Area

Protein Biochemistry

Images & Validation

−

Item 1 of 2

Tested Applications	SDS-PAGE
Application Notes	Rat IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Key Properties

−

Source	Rat
Biological Origin	Rat
Isotype	IgG F(ab')2
Purity	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	2.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Rat IgG F(ab')2 fragment, Rat IgG Fab2 fragment

Rat IgG F(ab')2 Fluorescein Antibody [orb346344]

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Source:	Rat

Sizes Available: 1 mg

Item 1 of 1

Rat IgG F(ab')2 Peroxidase Antibody [orb346349]

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Source:	Rat

Sizes Available: 1 mg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab; 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-PAGE Results of Rat F(ab')2 fragment (p/n orb2652742). Lane 1: Rat F(ab')2 Reduced 10 µg. Lane 2: Rat F(ab')2 Reduced 5 µg. Lane 3: Rat F(ab')2 Reduced 1 µg. Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Rat F(ab')2 Non-Reduced 10 µg. Lane 6: Rat F(ab')2 Non-Reduced 5 µg. Lane 7: Rat F(ab')2 Non-Reduced 1 µg. 4-20% Gel, Coomassie stained.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

View Protocol

Find More Protocols