You have no items in your shopping cart.
Rat IgG (H&L) Antibody Fluorescein Conjugated
SKU: orb347901
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, FC, FLISA, IF |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Gallus |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Rat IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Rat IgG and Rat Serum. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Chicken Anti-Rat IgG fluorescein Conjugated Antibody, Chicken Anti-Rat IgG Antibody FITC Conjugation
Similar Products
−- Item 1 of 2
Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed [orb347787]
FC, FLISA, IF, WB
Rat
Goat
Polyclonal
FITC
1 mg - Item 1 of 2
- Item 1 of 1
Fab Rat IgG (H&L) Antibody Fluorescein Conjugated [orb348496]
DOT, FC, FLISA, IF
Rat
Goat
Polyclonal
FITC
1 mg - Item 1 of 1
Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed [orb347857]
FC, FLISA, IF
Rat
Rabbit
Polyclonal
FITC
1 mg - Item 1 of 1
Rat IgG (H&L) Antibody Fluorescein Conjugated [orb347880]
FC, FLISA, IF, WB
Rat
Sheep
Polyclonal
FITC
2 mg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Dot Blot of Chicken anti-Rat IgG Antibody Fluorescein Conjugated. Antigen: Rat IgG. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: n/a. Secondary antibody: Chicken anti-Rat IgG Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT.
Western Blot of Anti-Rat IgG (H&L) (CHICKEN) Antibody (p/n orb347900). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (CHICKEN) Antibody (p/n orb347900) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb346892) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Rat IgG (H&L) Antibody Fluorescein Conjugated (orb347901)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days