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Description
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Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Rat IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rat IgG and Rat Serum. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 2.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Sheep Anti Rat IgG Antibody, Sheep Anti-Rat IgG Antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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