Rat IgG2a isotype control Fluorescein Antibody

SKU: orb343817

Description

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, FC
Application Notes	RAT IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Key Properties

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Source	Rat
Biological Origin	Rat
Isotype	IgG2a
Purity	RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG2a confirmed by Dot Blot.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rat Isotype Control, Rat IgG2a FITC isotype, RAT IgG2a subclass isotype control

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Rat IgG2a isotype control Fluorescein Antibody

Dot Blot results of Rat IgG2a Isotype Control Fluorescein Conjugated. Antigen: Rat IgG2a Isotype Control FITC Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT.

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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