Rat Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit

SKU: orb775108

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha(MIP1a).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MIP1 Alpha, CCL3 ELISA Kit, MIP1-A ELISA Kit, SCYA3 ELISA Kit, Chemokine C-C-Motif Ligand 3 ELISA Kit, Small Inducible Cytokine A3 ELISA Kit, Homologous To Mouse Mip-1a ELISA Kit, Tonsillar Lymphocyte LD78 Alpha Protein ELISA Kit

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Rat Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit

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UniProt

P50229

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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