Rat Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit

SKU: orb780037

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha(PPARgC1a).

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPARgC1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPARgC1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPARgC1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPARgC1a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.126 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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LEM6 ELISA Kit, PGC-1(alpha) ELISA Kit, PGC-1v ELISA Kit, PGC1 ELISA Kit, PGC1A ELISA Kit, PPARGC1 ELISA Kit, Ligand effect modulator 6 ELISA Kit

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Rat Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) ELISA Kit

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UniProt

Q9QYK2

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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