Rat Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

SKU: orb780344

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha(REG3a).

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REG3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REG3a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.127 ng/mL
Concentration	20 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

HIP ELISA Kit, INGAP ELISA Kit, PAP-H ELISA Kit, PAP1 ELISA Kit, PBCGF ELISA Kit, REG-III ELISA Kit, REG3 ELISA Kit, Pancreatitis-Associated Protein ELISA Kit, Hepatointestinal pancreatic protein ELISA Kit, Human proislet peptide ELISA Kit, Pancreatitis-associated protein 1 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Rat Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

Quick Database Links

UniProt

P35231

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols