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Rat Tissue Inhibitors of Metalloproteinase 4 (TIMP-4) ELISA Kit

SKU: orb1948896
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Description

Rat Tissue Inhibitors of Metalloproteinase 4 (TIMP-4) ELISA Kit

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Application Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TIMP-4. You can calculate the concentration of Rat TIMP-4 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityRat
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Assay TypeSandwich
Assay Time3.5H
Range0.16-10ng/mL
Sensitivity0.1 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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TIMP4

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Quick Database Links

Gene Symbol
TIMP-4
UniProt
P81556

UniProt Details

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No UniProt data available

Protocol Information

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48 T
$ 310.00
96 T
$ 430.00
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